Williams announced her withdrawal at a news conference at Roland Garros on Monday.
Rafael Nadal continued his bid for an 11th French Open title on Saturday by brushing aside home favourite Richard Gasquet to claim a 16th consecutive win over his childhood friend and reach the fourth round.
French No 1 Lucas Pouille, who had defeated Cameron Norrie in the second round, resumed his third-round match against Karen Khachanov trailing by two sets and the Russian completed a 6-3 7-5 6-3 success, which sees him meet second seed Alexander Zverev next.
"Of course I have won a lot, and it can maybe influence my opponents".
Although she was still in school, Dubreuil started to go to tournaments, funding her travels by selling pictures she took with her first camera, a Minolta X-300, of players including Steffi Graf, Andre Agassi and Boris Becker, for 10 francs (-1.50) each to fans.
"I played very well, I'm sorry for Richard, he is a good friend and a good person", Nadal said.
"I don't know what the others think". He hits the ball with big topspin.
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As is customary after a win, the player was interviewed by a court side presenter.
Third seed Marin Cilic reached the fourth round of a Grand Slam for the 20th time when he beat American Steve Johnson 6-3 6-2 6-4 in one hour, 34 minutes. I hope to be ready to play a good match.
Italy's Fabio Fognini made the last 16 for the second time with a 6-3, 4-6, 3-6, 6-4, 6-4 win over Britain's last man standing Kyle Edmund.
Diego Schwartzman joined countryman del Potro in the fourth round with an impressive 7-5, 6-3, 6-3 victory over Borna Coric.
There was, however, one upset in the women's event today as 25th seed Anett Kontaveit of Estonia beat two-time Wimbledon champion Petra Kvitova of the Czech Republic 7-6 (8-6), 7-6 (7-4).
There was more French despair in the delayed match between Belgian eighth seed David Goffin and France's Gael Monfils, who squandered four match points before losing a closely-fought encounter.
The 64th-ranked Mischa lost in the third round to 2017 US Open runner-up Kevin Anderson 6-1, 6-7 (3), 6-3, 7-6 (4) on Saturday. "Because I knew if I gave him only one opportunity to take the point, he will take it with his forehand". Cilic reached the final at Wimbledon a year ago and at the Australian Open in January.