"It's reality that today has been my last match for the national team", he told reporters.
The 34-year-old announced he had played his last game for his country following his country's shock penalty-shootout defeat in Moscow, adding: "Sometimes, the end is not as you dreamed it". A marvellous spell is over.
With 131 appearances, Iniesta was Spain's fourth-most-capped player, behind Iker Casillas, Sergio Ramos and Xavi Hernandez.
The former Barcelona midfielder has since announced his decision to retire from worldwide football.
However, Iniesta did accept coach Fernando Hierro's decision to leave him out of the starting XI.
'It's bad, it's tough.
Japan vs. Poland - Football Match Report
With scores level in the simultaneous Senegal and Colombia clash, the goal saw Japan fall to third spot in the group. He beat his marker at the far post and volleyed in a swerving free kick from Rafal Kurzawa .
"In the second half, we were trying to defend".
'On a personal level, it wasn't my dream farewell, but these things happen in life'.
Iniesta was later subbed on and also scored during the penalty shootout, but his fairy-tale finish to an illustrious Spain career wasn't to be.
But the way the draw has worked out, England have been presented with an unbelievable chance to potentially reach their first World Cup final since 1966.
Vladimir Putin called King Felipe VI of Spain after a FIFA World Cup Round of 16 match between the two national teams.
He was unable to inspire Spain to victory however as they were beaten 4-3 on penalties by the World Cup hosts. We will be back at big tournaments again.